abstract：:It is becoming increasingly clear that the adeno-associated virus 2 (AAV)-based vector system may prove to be useful for high-efficiency gene transfer in human cells as well as for potential gene therapy in humans. A recombinant AAV vector containing the gene for a human hematopoietic growth factor, granulocyte-macrop...

journal_title：Experimental hematology

authors： Luo F,Zhou SZ,Cooper S,Munshi NC,Boswell HS,Broxmeyer HE,Srivastava A

更新日期：1995-11-01 00:00:00

abstract：:We created fully and stable xenogeneic hematopoietic chimeras in "poorly concordant" rat-mouse strain combinations defined by their high histocompatibility-antigen disparity and by the high titer of mouse-serum natural cytotoxic antibodies (NcAb) to rat donor bone marrow cells (BMC). Recipients were adult male (C57BL/...

journal_title：Experimental hematology

authors： Spach C,Lucchiari N,Bureaud N,Motta R

更新日期：1995-10-01 00:00:00

abstract：:Activation of the N-ras gene via coding region mutations has been previously documented in 25% of patients with acute myeloid leukemia (AML). Since overexpression of normal N-ras is also transforming in vitro, the N-ras promoter of AML patients was analyzed for the presence of promoter mutations that may affect expres...

journal_title：Experimental hematology

authors： Thorn J,Molloy P,Iland H

更新日期：1995-09-01 00:00:00

abstract：:The present studies evaluated effects of recombinant human (rhu) and murine (rmu) flt3 ligand (flt3-L) on colony formation by subsets of myeloid stem and progenitor cells present in low-density (LD) and cell-sorted CD34 hu cord blood (CB) and bone marrow (BM) cells and unseparated mu BM cells. By itself, flt3-L had we...

journal_title：Experimental hematology

authors： Broxmeyer HE,Lu L,Cooper S,Ruggieri L,Li ZH,Lyman SD

更新日期：1995-09-01 00:00:00

abstract：:The present studies have investigated, for the first time, the synergistic effects of interleukin-4 (IL-4) and IL-11 on the growth of single murine bone marrow progenitor cells. These studies suggest that IL-4 and IL-11 are synergistic hematopoietic growth factors, enhancing colony formation of bone marrow progenitors...

journal_title：Experimental hematology

authors： Jacobsen FW,Keller JR,Ruscetti FW,Veiby OP,Jacobsen SE

更新日期：1995-08-01 00:00:00

abstract：:To characterize hematopoietic cell biology, many investigators have used protocols that enrich for primitive hematopoietic stem cells (PHSC). In this study, we quantified the long-term repopulating ability (LTRA) of enriched and discarded fractions of PHSC from day-14 murine fetal liver using the competitive repopulat...

journal_title：Experimental hematology

authors： Jordan CT,Astle CM,Zawadzki J,Mackarehtschian K,Lemischka IR,Harrison DE

更新日期：1995-08-01 00:00:00

abstract：:Together, interleukin-1 alpha (IL-1 alpha) and IL-1 beta primed human neutrophils for enhanced release of superoxide (O2-) stimulated by chemotactic peptide, chemokine, and plant lectin, and alone, each triggered O2- release in a dose-dependent manner. The maximal priming and triggering effect was obtained by high con...

journal_title：Experimental hematology

authors： Yagisawa M,Yuo A,Kitagawa S,Yazaki Y,Togawa A,Takaku F

更新日期：1995-07-01 00:00:00

abstract：:As neutropenia is a common side effect of treatment with 2-chlorodeoxyadenosine (2-CdA), we investigated the myelosuppressive action of 2-CdA in Dexter-type human long-term bone marrow cultures (LTBMCs). LTBMCs were incubated with varying doses of 2-CdA (5 to 20 nM/L) during the first week. At 20 and 10 nM/L 2-CdA, we...

journal_title：Experimental hematology

authors： Petzer AL,Geisen FH,Bilgeri R,Zilian U,Haun M,Herold M,Braunsteiner H,Konwalinka G

更新日期：1995-06-01 00:00:00

abstract：:The development of pluripotential hematopoietic stem cells (PHSC) requires the continuous support provided by the bone tissue and bone marrow (BM) stromal cells. The basic rule of spatial and temporal organization of the distinct stromal cells and differentiating hematopoietic cells in the course of development, regen...

journal_title：Experimental hematology

authors： Blazsek I,Liu XH,Anjo A,Quittet P,Comisso M,Kim-Triana B,Misset JL

更新日期：1995-04-01 00:00:00

abstract：:We evaluated the expansion capacity of untreated and 5-fluorouracil (5-FU)-resistant peripheral blood stem cells (PBSC) after 7-day incubation with interleukin-1 (IL-1) plus IL-3 plus stem cell factor (SCF) or with medium alone. We found a significant increase in the proportion of CD34+ cells in the PBSC fraction resi...

journal_title：Experimental hematology

authors： Rice A,Boiron JM,Barbot C,Dupouy M,Dubosc-Marchenay N,Dumain P,Lacombe F,Reiffers J

更新日期：1995-04-01 00:00:00

abstract：:The production of interferon-alpha (IFN-alpha) by normal human peripheral blood mononuclear cells (PBMNC) was studied using polyclonal antipeptide antibodies designed to react either with all IFN-alpha subtypes or with individual subtypes IFN-alpha 2 or IFN-alpha 4. In this study, we demonstrate the detection of intra...

journal_title：Experimental hematology

authors： Greenway AL,Hertzog PJ,Devenish RJ,Linnane AW

更新日期：1995-03-01 00:00:00

abstract：:The presence of granulocyte colony-stimulating factor receptor (G-CSFR) in CD34+ hematopoietic progenitors is demonstrated by flow cytometry. Cord blood mononuclear cells (MNCs) contain about 1.09 +/- 0.2% CD34+ cells, 0.32 +/- 0.1% CD34+/G-CSFR+ (G-CSFR+) cells, and 0.77 +/- 0.1% CD34+/G-CSFR- (G-CSFR-) cells. The co...

journal_title：Experimental hematology

authors： Shimoda K,Okamura S,Harada N,Kubota A,Iwasaki H,Ohno Y,Niho Y

更新日期：1995-03-01 00:00:00

abstract：:In a previous report, we described a method to generate cell lines derived from stromal colonies (colony-derived cell lines [CDCL]). In a first step, colonies were obtained from plating cells from adherent layers of human long-term bone marrow cultures (LTBMC) in methyl-cellulose in the presence of interleukin-1 beta ...

journal_title：Experimental hematology

authors： Li J,Sensebé L,Hervé P,Charbord P

更新日期：1995-02-01 00:00:00

abstract：:Bone marrow endothelial cells are likely to play an important role in the homing of hematopoietic progenitor cells. In view of analyzing the interactions between endothelial cells and hematopoietic progenitor cells, we studied several methods of isolating endothelial cells from human bone marrow, including fluorescenc...

journal_title：Experimental hematology

authors： Schweitzer CM,van der Schoot CE,Dräger AM,van der Valk P,Zevenbergen A,Hooibrink B,Westra AH,Langenhuijsen MM

更新日期：1995-01-01 00:00:00

abstract：:This report presents the results of studies using long-term bone marrow cultures (LTBMC) of human bone marrow cells to investigate the effect of HIV-1 on in vitro hematopoiesis. Confluent stromal cell layers established from human bone marrow cells were irradiated to eliminate residual hematopoietic progenitor cells a...

journal_title：Experimental hematology

authors： Schwartz GN,Kessler SW,Rothwell SW,Burrell LM,Reid TJ,Meltzer MS,Wright DG

更新日期：1994-12-01 00:00:00

abstract：:Growth and differentiation of hematopoietic stem cells occur in close contact with the cells and extracellular-matrix (ECM) proteins of the hematopoietic microenvironment. We observed the role of fibronectin, a matrix glycoprotein proposed to be involved in the attachment of hematopoietic cells and in the binding of s...

journal_title：Experimental hematology

authors： van der Sluijs JP,Baert MR,Ploemacher RE

更新日期：1994-12-01 00:00:00

abstract：:In a nonrandomized study, hematopoietic recovery kinetics were evaluated in 98 consecutive patients who underwent high-dose chemotherapy without total body irradiation (TBI) and autologous peripheral blood stem cell transplantation (PBSCT). Fifty-three patients received recombinant human granulocyte colony-stimulating...

journal_title：Experimental hematology

authors： Suzue T,Takaue Y,Watanabe A,Kawano Y,Watanabe T,Abe T,Kuroda Y,Matsushita T,Kikuta A,Iwai A

更新日期：1994-11-01 00:00:00

abstract：:In a selective culture system, the in vitro generation of terminal deoxynucleotidyl transferase positive (TdT+) B cell progenitors from rat, mouse, and human bone marrow (BM) is characterized by initial lymphoid colonization of a mouse BM feeder layer. In the present study, four sequentially appearing subsets of B cel...

journal_title：Experimental hematology

authors： McKenna SD,Medlock ES,Greiner DL,Goldschneider I

更新日期：1994-11-01 00:00:00

abstract：:We have reported modulation, by cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) and by hormonal cyclic-adenosine-monophosphate (cAMP) agonists, of hematopoietic growth factor production in the murine marrow adherent cell line +/+(-)1.LDA11. Previously, we reported that increased in...

journal_title：Experimental hematology

authors： Derigs HG,Reifel-Miller A,Kaushansky K,Hromas RA,Boswell HS

更新日期：1994-08-01 00:00:00

abstract：:The hematologic effects of recombinant human interleukin-6 (rhIL-6) were studied in dogs exposed to a total-body irradiation (TBI) of 2.4 Gy. IL-6 was administered over a period of 14 days at a daily dose of 18 micrograms/kg by single subcutaneous injection. Treatment was started 1 day after TBI. The data obtained for...

journal_title：Experimental hematology

authors： Selig C,Kreja L,Müller H,Seifried E,Nothdurft W

更新日期：1994-07-01 00:00:00

abstract：:The high affinity Fc gamma receptor, Fc gamma RI, is unique among the three classes of macrophage Fc gamma receptors not only in its affinity for IgG, but also in the structure of its cytoplasmic domain. Fc gamma RIIA and the gamma subunit of Fc gamma RIIIA have tyrosine-containing motifs within their cytoplasmic doma...

journal_title：Experimental hematology

authors： Indik ZK,Hunter S,Huang MM,Pan XQ,Chien P,Kelly C,Levinson AI,Kimberly RP,Schreiber AD

更新日期：1994-07-01 00:00:00

abstract：:A thymic stromal cell line with a medullary phenotype (Z210R.1) supported the differentiation of surface IgM+ B cells when cocultured with fetal liver cells in vitro. Conditioned medium (CM) from this cell line supported the long-term growth of a B cell line (NAG8/7) isolated from cocultures and enhanced the prolifera...

journal_title：Experimental hematology

authors： Friend SL,Hosier S,Nelson A,Foxworthe D,Williams DE,Farr A

更新日期：1994-03-01 00:00:00

abstract：:Effects of thyroid hormones on the production of erythropoietin (Epo) were investigated in isolated perfused rat kidneys and in the human hepatoma cell line, HepG2. Epo protein was measured by radioimmunoassay. L-triiodothyronine and L-thyroxine stimulated hypoxia-induced Epo formation both in the kidney and in HepG2 ...

journal_title：Experimental hematology

authors： Fandrey J,Pagel H,Frede S,Wolff M,Jelkmann W

更新日期：1994-03-01 00:00:00

abstract：:A method is described that permits the removal of hematopoietic cells and macrophages from mouse bone marrow cultures. The method is based on the difference in effect of extracellular ATP4- ions (ATP in the absence of divalent, complexing cations) on cells of hematopoietic origin, including macrophages, and of nonhema...

journal_title：Experimental hematology

authors： Modderman WE,Vrijheid-Lammers T,Löwik CW,Nijweide PJ

更新日期：1994-02-01 00:00:00

abstract：:We have investigated the effect of hematopoietic cytokines on the survival of severe combined immune-deficient (SCID) mice that received a high dose of radiation. In this study, female SCID mice were irradiated at doses ranging from 500 to 600 cGy and then transplanted with 2 x 10(6) male Balb/c marrow cells. Groups o...

journal_title：Experimental hematology

authors： Wu DD,Bosch B,Keating A

更新日期：1994-02-01 00:00:00

abstract：:The immediate hematopoietic response to 3'-azido-3'-deoxythymidine (AZT) was characterized in an unperturbed and regenerating mouse marrow model to identify the in vivo hematopoietic targets of AZT and test whether AZT toxicity is dependent on the proliferative activity of the hematopoietic targets. B6D2F1 mice receiv...

journal_title：Experimental hematology

authors： Scheding S,Media JE,Nakeff A

更新日期：1994-01-01 00:00:00

abstract：:The bcr/abl transcript is specifically expressed in the hematopoietic cells of patients with chronic myeloid leukemia (CML). The Haseloff and Gerlach model was used to design a ribozyme targeted to this transcript. When tested against a synthetic substrate covering the translocation sequence, the ribozyme exhibited si...

journal_title：Experimental hematology

authors： Wright L,Wilson SB,Milliken S,Biggs J,Kearney P

更新日期：1993-12-01 00:00:00

abstract：:Despite its growing use as a marker of proliferation, the relationship between expression of the nuclear antigen Ki67 and other indices of proliferation in acute myelogenous leukemia (AML) has not been elucidated. In this study, short-term primary suspension cultures of human AML bone marrow cells were used to compare...

journal_title：Experimental hematology

authors： Gore SD,Weng LJ,Burke PJ

更新日期：1993-12-01 00:00:00

abstract：:Delayed platelet recovery and altered marrow megakaryocytopoiesis was observed in highly irradiated mice following transplantation with 1000 lineage-negative, stem cell antigen-positive (Lin-Sca-1+) cells. Thirty days after transplantation, the mice were thrombocytopenic. Normal platelet levels were reestablished by 9...

journal_title：Experimental hematology

authors： Bradford G,Williams N,Barber L,Bertoncello I

更新日期：1993-12-01 00:00:00

abstract：:Peritoneal injection of thioglycollate medium (TM) to mice results in a dramatic increase in total number of peritoneal macrophages within 48 to 72 hours. Unlike resident macrophages, a fraction (10 to 20%) of these newly arrived young macrophages, designated as macrophage colony-forming cells (M-CFC), are highly prol...

journal_title：Experimental hematology

authors： Chen B,Chou TH,Sensenbrenner L

更新日期：1993-11-01 00:00:00

abstract：:Seven patients received cancer chemotherapy with high-dose cyclophosphamide (HD-CTX) associated with either recombinant human granulocyte colony-stimulating factor (rhG-CSF), rh interleukin-3 (rhIL-3), rh granulocyte-macrophage CSF (rhGM-CSF) plus rh erythropoietin (rhEpo), rhIL-3 plus rhGM-CSF, or rhIL-3 plus rhG-CSF...

journal_title：Experimental hematology

authors： Siena S,Bregni M,Bonsi L,Sklenar I,Bagnara GP,Bonadonna G,Gianni AM

更新日期：1993-11-01 00:00:00

abstract：:It has been reported that bone marrow and serum of patients with aplastic anemia or chronic myeloproliferative disorders contain an abnormal concentration of cytokines. In the present study, we tried to isolate mouse bone marrow stromal cell lines that were stably transformed with a variety of cytokine genes and that ...

journal_title：Experimental hematology

authors： Nakano T,Otsuka T,Ogo T,Niiro H,Kuga S,Zipori D,Furukawa Y,Niho Y

更新日期：1993-10-01 00:00:00

abstract：:Thrombocytopenia is an important clinical problem for many acquired immunodeficiency syndrome (AIDS) patients. Recently, the utility of recombinant cytokines in alleviating the hematopoietic complications of AIDS and AIDS therapy has been evaluated. The newly cloned cytokine stem cell factor (SCF) has been demonstrate...

journal_title：Experimental hematology

authors： Chow FP,Zsebo K,Hamburger AW

更新日期：1993-08-01 00:00:00

abstract：:Previous studies in animal models have suggested that bone marrow-derived interleukin-2 (IL-2) activated natural killer (A-NK*) cells can be used to eliminate residual leukemic cells both in vivo and in vitro. As part of initial studies to develop a clinical protocol to exploit this phenomenon in combination with cult...

journal_title：Experimental hematology

authors： Klingemann HG,Deal H,Reid D,Eaves CJ

更新日期：1993-08-01 00:00:00

abstract：:Vascular endothelial cells can affect hematopoiesis by releasing granulocyte-macrophage colony-stimulating factor (GM-CSF). In the present study, we show that GM-CSF production in murine aorta-derived vascular endothelial cells is regulated by two cytokines:interleukin-1 (IL-1), which induces the production of GM-CSF,...

journal_title：Experimental hematology

authors： Akahane K,Pluznik DH

更新日期：1993-07-01 00:00:00

abstract：:Protein kinase activities are involved in cellular proliferation and differentiation, and inhibitors of these activities are useful for studying the mechanisms of induction of differentiation. We found that staurosporine, an inhibitor of protein kinase activities, induced morphological differentiation of human myelobl...

journal_title：Experimental hematology

authors： Makishima M,Honma Y,Hozumi M,Sampi K,Motoyoshi K,Nagata N,Hattori M

更新日期：1993-07-01 00:00:00

abstract：:Phagocytic cells are characterized by their ability to generate superoxide anions upon activation by appropriate stimuli. UM384, a myelomonocytic cell line, was shown to be defective in this oxidase activity as measured by nitroblue tetrazolium or cytochrome c reduction. Cytochrome b558, a unique pigment present in ph...

journal_title：Experimental hematology

authors： Champelovier P,Laporte F,Verhoeven AJ,Hilarius P,de Klein A,Revol C,Seigneurin D,Kolodie L

更新日期：1993-07-01 00:00:00

abstract：:Cells of the murine interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) factor-dependent line, FDC-P1, express the tyrosine kinase receptor, c-kit. The ligand for c-kit, steel factor (SLF), encoded by the steel (Sl) locus, is produced as both membrane-bound and soluble forms by fibroblast...

journal_title：Experimental hematology

authors： Caruana G,Ashman LK,Fujita J,Gonda TJ

更新日期：1993-06-01 00:00:00

abstract：:Are the stem cells that survive graded doses of ionizing radiation as effective in restoring hematopoiesis in irradiated mice as transfused nonirradiated stem cells? This question was addressed by determining animal dose mortality and 10-day colony-forming units (CFU-S) survival curves and then replotting the percent ...

journal_title：Experimental hematology

authors： Cronkite EP,Inoue T,Hirabayashi Y,Bullis J

更新日期：1993-06-01 00:00:00

abstract：:Serum erythropoietin (Epo) levels are depressed in anemic AIDS patients relative to controls. The basis for abnormal regulation of Epo has not been defined. The hepatoma cell line Hep3B produces Epo in response to hypoxia and serves as a model for the study of Epo regulation. Hep3B cells are infectible with human immu...

journal_title：Experimental hematology

authors： Wang Z,Goldberg MA,Scadden DT

更新日期：1993-05-01 00:00:00